Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: Typical expression images of immunochemistry staining in tissue microarrays. (A and B) Low expression of CD68 in MIA tissues. Original magnification, (A) ×100 and (B) ×200. (C and D) High expression of CD68 in LPA tissues. Original magnification, (C) ×100 and (D) ×200. (E) Negative expression of IL-6 in MIA tissues. Original magnification, ×200. (F) Positive expression of IL-6 in LPA tissues. Original magnification, ×200. (G) Negative expression of CSF-1 in MIA tissues. Original magnification, ×200. (H) Positive expression of CSF-1 in LPA tissues. Original magnification, ×200. (I) High E-cadherin expression in MIA tissues. Original magnification, ×100. (J) Low E-cadherin expression in LPA tissues. Original magnification, ×100. (K) High Snail expression in LPA tissues. Original magnification, ×100. (L) High MMP-2 expression in LPA tissues. Original magnification, ×100. CD, cluster of differentiation; MIA, minimally invasive adenocarcinoma; LPA, lepidic predominant adenocarcinoma; CSF, colony-stimulating factor; IL, interleukin; MMP, metalloproteinase.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: Expressing, Staining

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) Kaplan-Meier analysis of overall survival for the combination of CD68, CSF-1 and IL-6. (B) Kaplan-Meier analysis of disease-free survival for the combination of CD68, CSF-1 and IL-6. CSF-1, colony-stimulating factor-1; CD, cluster of differentiation; IL, interleukin,

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques:

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) OS in patients with AIS/MIA in the CD68+CSF-1+IL-6+ group compared with other groups, including the CD68-, CD68+CSF-1+IL-6-, CD68+CSF-1-IL-6+ and CD68+CSF-1-IL-6- groups. (B) DFS in patients with AIS/MIA in the CD68+ CSF-1+ IL-6+ group compared with other groups (C) Comparison of OS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. (D) Comparison of DFS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. *Others include the CD68+CSF-1+IL-6- group, the CD68+CSF-1-IL-6+ group, the CD68+CSF-1-IL-6- group and the CD68- group. AIS, adenocarcinoma in situ ; MIA, minimally invasive adenocarcinoma; CD, cluster of differentiation; CSF, colony-stimulating factor; IL, interleukin; OS, overall survival; DFS, disease-free survival.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: In Situ

Journal: Frontiers in Neurology

Article Title: Immune Response After Cochlear Implantation

doi: 10.3389/fneur.2020.00341

Figure Lengend Snippet: Immunohistochemistry with IL-6 in the scala tympani along the basal turn of the cochlea, according to treatment. NER group (A–E) ; DER group (F–H) . Arrows indicate staining inside the fibrotic tissue; black arrowheads indicate stained cells; white arrowheads indicate stained FGB. S, rod site; Scale bars, 100 μm (A,F) , 20 μm (B,C,G) , and 10 μm (D,E,H) .

Article Snippet: Sections were then incubated with the primary antibody at the concentrations 1:250 for IL-6 (bs-0782R, Bioss Inc, Woburn, Massachusetts, USA) and 1:200 for TNFalfa (ab1793, abcam, San Francisco, USA) overnight at 4°C.

Techniques: Immunohistochemistry, Staining

Journal: Frontiers in Neurology

Article Title: Immune Response After Cochlear Implantation

doi: 10.3389/fneur.2020.00341

Figure Lengend Snippet: Immunohistochemistry with IL-6 in the cochleostomy site after 60 days, according to treatment. NER group (A–E) ; DER group (F,G) . Arrows indicate staining inside the fibrotic tissue; black arrowheads indicate stained cells; white arrowheads indicate stained FGB. S, rod site; Scale bars, 100 μm (A,F) , 50 μm (B,C,G) , and 10 μm (D,E) .

Article Snippet: Sections were then incubated with the primary antibody at the concentrations 1:250 for IL-6 (bs-0782R, Bioss Inc, Woburn, Massachusetts, USA) and 1:200 for TNFalfa (ab1793, abcam, San Francisco, USA) overnight at 4°C.

Techniques: Immunohistochemistry, Staining

Journal: Adipocyte

Article Title: Effects of SPARCL1 on the proliferation and differentiation of sheep preadipocytes

doi: 10.1080/21623945.2021.2010901

Figure Lengend Snippet: Primer sequences for a quantitative real-time polymerase chain reaction

Article Snippet: IL6 , Rabbit polyclonal antibody , 1:1000 , Bioss, bs-4587 R.

Techniques: Sequencing

Journal: Adipocyte

Article Title: Effects of SPARCL1 on the proliferation and differentiation of sheep preadipocytes

doi: 10.1080/21623945.2021.2010901

Figure Lengend Snippet: The information of antibodies used in Western blot

Article Snippet: IL6 , Rabbit polyclonal antibody , 1:1000 , Bioss, bs-4587 R.

Techniques: Western Blot

Journal: Adipocyte

Article Title: Effects of SPARCL1 on the proliferation and differentiation of sheep preadipocytes

doi: 10.1080/21623945.2021.2010901

Figure Lengend Snippet: SPARCL1 affects the Wnt/β-catenin pathway related genes (a) GSK3β, FZD8, Wnt10b, β-catenin, IL6 expression patterns in preadipocyte differentiation; These genes expression is the highest at day 6(** P < 0.01). (b) Inhibition and overexpression with the SPARCL1 gene affect the mRNA expression of GSK3β, FZD8, Wnt10b, β-catenin, IL6 . (c) Inhibition and overexpression with the SPARCL1 gene affect the protein expression of GSK3β, FZD8, Wnt10b, β-catenin, IL6. (d) Densitometric analyses of the Western blots. The first set of data in the figure is named ‘a’. Lowercase letters different from a represent significant data differences between groups ( P < 0.05), named ‘b, c, and d’ in that order, and the same lowercase letters represent non-significant data differences( P > 0.05)

Article Snippet: IL6 , Rabbit polyclonal antibody , 1:1000 , Bioss, bs-4587 R.

Techniques: Expressing, Inhibition, Over Expression, Western Blot

Journal: Frontiers in Immunology

Article Title: MSC therapy ameliorates experimental gouty arthritis hinting an early COX-2 induction

doi: 10.3389/fimmu.2023.1193179

Figure Lengend Snippet: Ad-MSC modulates pro- and anti-inflammatory cytokine profile in synovial membranes of arthritic rabbits. Representative western blot of pro-inflammatory cytokines (A) COX-2, TNF, IL-6 and M2 anti-inflammatory cytokines levels (B) IL-10 and TGF-β. EZ blue staining was used as protein loading control and to normalize the results, which are expressed as a fold-change of the Control group. Bars show the mean and SEM. COX-2, Ciclooxygenase-2; IL, interleukin; TGF-β, tumor growth factor-β, TNF-α, tumor necrosis factor α; MSC, mesenchymal stem cells; MSU, monosodium urate.

Article Snippet: The following primary antibodies were applied overnight at 4°C: anti-human COX-2 (Santa Cruz Biotechnology, Dallas TX, USA), anti-rabbit IL-6, anti-rabbit TNF, anti-rabbit IL-10, anti-rabbit TGF-β (all from Cloud-Clone Corp; 1/250 dilution); anti-human NLRP3 (AdipoGen, Liestal, Switzerland; 1/1000 dilution); anti-human Caspase-1 (Thermo Fisher Scientific, IL, USA; 1/500 dilution), anti-rabbit IL-18 and IL-1β antibodies (Cloud-Clone Corp, Houston TX, USA; 1/250 dilution).

Techniques: Western Blot, Staining

Journal: International Journal of Molecular Sciences

Article Title: Novel Medicinal Mushroom Blend as a Promising Supplement in Integrative Oncology: A Multi-Tiered Study using 4T1 Triple-Negative Mouse Breast Cancer Model

doi: 10.3390/ijms21103479

Figure Lengend Snippet: Primary/secondary antibodies and respective dilution used for immunocytochemical experimental procedures.

Article Snippet: Primary antibodies , Anti-Interleukin-6 (M-19) , Purified antibody raised against a peptide mapping at the C-terminus of IL-6 of mouse origin , Santa Cruz Biotechnology (Santa Cruz, CA, USA), Goat polyclonal IgG, Cat# sc-1265, RRID: AB_2127470 , 1:100.

Techniques: Purification, Plasmid Preparation